VIRULENCE OF ENTOMOPATHOGENIC NEMATODE TO PUPAE AND ADULTS OF Drosophila suzukii IN LABORATORY

Andressa Lima de Brida, Silvia Renata Siciliano Wilcken, Luis Garrigós Leite, Flávio Roberto Mello Garcia

Resumo


Drosophila suzukii is considered one of the most important pests of fruit farming. Due to its rapid expansion, control alternatives of this fly should be investigated. The use of entomopathogenic nematodes (EPNs) represents an important tool in the control. This study aimed to evaluate the virulence of EPNs isolates in pupae and the repercussion in adults of D. suzukii in laboratory. The experiment was conducted in a completely randomized design with five treatments and five replicates. Each plot consisted of a Petri dish lined with two sheets of filter paper. The isolates Heterorhabditis amazonensis IBCBn 24, Heterorhabditis indica IBCBn 05, Steinernema carpocapsae IBCBn 02 and Steinernema feltiae IBCBn 47 were inoculated into 2 mL at the concentration of 1,000 infective juveniles IJs/mL. The control treatment consisted of 2 mL distilled water. After inoculation, five pupae of D. suzukii were placed in the Petri dishes, which were then sealed and stored in a BOD climate chamber at 26 ± 1ºC, 70 ± 10% RH in the dark. Assessments were performed daily until the emergence of adults. Dead pupae and adults were dissected for the observation and quantification of IJs. The isolates, H. indica IBCBn 05, H. amazonensis IBCBn 24, S. carpocapsae IBCBn 02 and S. feltiae IBCBn 47, infected and made unviable 35.0, 16.0, 13.0 and 43.0% in pupae and 47.0, 80.0, 84.0 and 57.0 % in adults of D. suzukii. H. indica IBCBn 05 obtained the highest number of IJs produced in pupae and adults, 35.0 and 125.0.


Texto completo:

PDF

Referências


AREFIN B.; KUCEROVA L.; DOBES P.; MARKUS R.; STRNAD H.; WANG Z.; HYRSL, P.; ZUROVEC, M.; THEOPOLD, U. Genome-wide transcriptional analysis of Drosophila larvae infected by entomopathogenic nematodes shows involvement of complement, recognition and extracellular matrix proteins. Journal of Innate Immunity. v.6, n.2, p.192–204, 2014. DOI: https://doi.org/10.1159/00035373.4.

ATTALA, A.; FATIMA, A.; EWEIS, M.A. Preliminary investigation on the utilization of entomopathogenic nematodes as biological control agents against the peach fruit fly, Bactrocera zonata (Saunders) (Diptera: Tephritidae). Egyptian Journal of Agricultural Research. v.80, n.3, p.1045-1053, 2002.

BERRY, J. A.Pest risk assessment: Drosophila suzukii: spotted wing drosophila (Diptera: Drosophilidae) on fresh fruit from the USA. Ministry for Primary Industries. New Zealand: MPI Technical Paper, 2012. 48p.

BRIDA, A.L; WILCKEN, S.R. LEITE, L. G. Influência do substrato e da luminosidade na infecção de nematoides entomopatogênicos em Galleria mellonella (Lepidoptera: Pyralidae). Revista Cientifica Rural. v.20, n.2, p.91-101, 2018. DOI: https://doi.org/10.30945/rcr-v20i2.307.

BRIDA, A. L; ROSA, J. M. O.; OLIVEIRA, C. M. G.; CASTRO, B. M. C.; SERRÃO, J. E.; ZANUNCIO, J. C.; LEITE, L. G.; WILCKEN, S. R. S. Entomopathogenic nematode in agricultural in Brazil. Scientific Reports. v.7, n.45254, p.1-9, 2017. DOI: https://dx.doi.org/10.1038%2Fsrep45254.

CASTILLO, J.C.; REYNOLDS, S.E.; ELEFTHERIANOS, I. Insect immune responses to nematode parasites. Trends in Parasitolology. v.27, n.12, p.537–547, 2011. DOI: http://dx.doi.org/10.1016/j.pt.2011.09.001.

CICHE, T.A.; ENSIGN, J.C. For the insect pathogen Photorhabdus luminescens, which end of a nematode is out?. Applied Environmental Microbiology. v. 69, n.4, p.1890-1897, 2003. DOI: http://dx.doi.org/10.1128/AEM.69.4.1890-1897.2003.

CINI, A.; IORIATTI, C.; ANFORA, G. A review of the invasion of Drosophila suzukii in Europe and a draft research agenda for integrated pest management. Bulletin of Insectology. v.65, n.1, 149–160, 2012..

CUTHBERTSON, A.G.S.; COLLINS, D.A.; BLACKBURN, L.F.; AUDSLEY, N.; BELL, H.A. Preliminary screen ing of potential control products against Drosophila suzukii. Insects. v.5, n.2, p.488–498, 2014. DOI: http://dx.doi.org/10.3390/insects5020488.

CUTHBERTSON, A.G.S.; AUDSLEY, N. Further screening of entomopathogenic fungi and nematodes as control agents for Drosophila suzukii. Insects. v.7, n.2, p.1–9, 2016. DOI: http://dx.doi.org/10.3390/insects7020024.

DALTON, P.T.; WALTON, V.M.; SHEARER, P.W.; WALSH, D.B.; CAPRILE, J.; ISAACS, R. Laboratory survival of Drosophila suzukii under simulated winter conditions of the Pacific Northwest and seasonal field trapping in five primary regions of small and stone fruit production in the United States. Pest Management Science. v.67, n.11, p.1368-1374, 2011. DOI: http://dx.doi.orr/10.1002/ps.2280.

DEPRÁ, M.; POPPE, J.L.; SCHMITZ, H.J.; TONI, D.C.; VALENTE, V.L.S. The first records of the invasive pest Drosophila suzukii in the South American continent. Journal of Pest Science. v. 87, n.1, p.379–383, 2014. DOI:https://doi.org/10.1007/s10340-014-0591-5.

DOS SANTOS, L.A.; MENDES, M.F.; KRUGER, A.P.; BLAUTH, M.L., GOTTSCHALK, M.S.; GARCIA, F.R.M. Global potential distribution of Drosophila suzukii (Diptera, Drosophilidae). Plos one. v.12, n.13, p.1-13, 2017. DOI: https://doi.org/10.1371/journal.pone.0174318.

FERREIRA, D. F. Sisvar: a computer statistical analysis system. Ciência e Agrotecnologia. v.35, n.6, p.1039-1042, 2011. DOI: http://dx.doi.org/10.1590/S1413-70542011000600001.

FOELKEL, E.; MONTEIRO, L.B.; VOSS, M. Virulence of nematodes against larvae of the south-American fruit fly in laboratory using soil from Porto Amazonas, Paraná, Brazil, as substrate. Ciência Rural. v.46, n.3, p.405–410, 2016. DOI: http://dx.doi.org/10.1590/0103-8478cr20150370.

FUJIMOTO, A.; LEWIS, E.E.; COBANOGLU, G.; KAYAL, H.K. Dispersal infectivity and sex ratio of early- or late-emerging infective juveniles of the entomopathogenic nematode Steinernema carpocapsae. Journal of Nematology. v.39, n.4, p.333–337. 2007..

GREWAL, P.S. Anhydrobiotic potential and long-term storage of entomopathogenic nematodes (Rhabditida: Steinernematidae). International Journal for Parasitology. v.30, n.9, p.995-1000, 2000. DOI: https://dx.doi.org/10.1016/S0020-7519(00)00080-1.

HALLEM, E.A.; RENGARAJAN, M.; CICHE, T.A.; STERNBERG, P.W.. Nematodes, bacteria, and flies: a tri partite model for nematode parasitism. Current Biology. v. 17, n. 10, p.898–904, 2007. DOI: http://dx.doi.org/10.1016/j.cub.2007.04.027.

HUSER, M. A historic account of the invasion of Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) in the continental United States, with remarks on their identification. Pest Management Science. v.67, n.11, p.1352–1357, 2011. DOI: http://dx.doi.org/10.1002/ps.2265.

KUCHARSKA, K.; KUCHARSKA, D.; ZAJDEL, B. Bacteria Xenorhabdus and Photorhabdus, entomopathogenic nematodes and insects -Their role in the complex symbiont-parasite-host relationship. Postepy Mikrobiol. v.54, p.154-164, 2015.

LEWIS, E.E.; CAMPBELL, J.; GRIFFIN, C.; KAYA, H.K.; PETERS, A. Behavioral ecology of entomopathogenic nematodes. Biological Control. v. 38, n.1, p.66–79, 2006. DOI: https://doi.org/10.1016/j.biocontrol.2005.11.007.

LINDEGREN, J.E.; VAIL, P.V. Susceptibility of mediterranean fruit fly, melon fly, and oriental fruit fly (Diptera: Tephritidae) to the entomogenous nematode Steinernema feltiae in laboratory tests. Environmental Entomology. v.15, n.3, p.465–468, 1986. DOI: https://doi.org/10.1093/ee/15.3.465.

MALLON, E. B.; BROCKMANN, A.; SCHMID-HEMPEL, P. Imunne response inhibits associative learning in insects. Proceedings of the Royal Society Biological Sciences. v.270, p.2471–2473, 2003. DOI: http://dx.doi.org/10.1098/rspb.2003.2456.

MEKETE, T.; GAUGLER, R.; NGUYEN, K.B.; MANDEFRO, W.; TESSERA, M. Biogeography of entomopathogenic nematodes in Ethiopia. Nematropica. v.35, n.1, p.31–36, 2005.

POINAR, G.O.; GREWAL, P.S. History of entomopathogenic nematology. Journal of Nematology. v.44, n.2, p.153–161, 2012.

RENKEMA, J.M.; WRIGHT, D.; BUITENHUIS, R.; HALLETT, R.H. Plant essential oils and potassium metabisulfite as repellents for Drosophila suzukii (Diptera : Drosophilidae). Scientific Reports. v.6, p.1–10, 2016. DOI: http://dx.doi.org/10.1038/srep21432.

SILVA, A.C.; BATISTA FILHO, A.; LEITE, L.G.; TAVARES, F.M.; RAGA, A.; SCHMIDT, F.S. Efeito de nematoides entomopatogênicos na mortalidade da mosca-do-mediterrâneo, Ceratitis capitata, e do gorgulho-da-goiaba, Conothachelus psidii. Nematologia Brasileira. v.34, n.1, p.31-40, 2010.

STRAND, M. R. The insect cellular immune response. Insect Science. v.15, n.1, p.1-14, 2008. DOI: http://dx.doi.org/10.1111/j.1744-7917.2008.00183.x.

VALLET-GELY, I.; LEMAITRE, B.; BOCCARD, F. Bacterial strategies to overcome insect defences. Nature Reviews Microbiology. v.6, p.302-313, 2008. DOI: http://dx.doi.or/10.1038/nrmicro1870.

VILELA, C.R.; MORI, L. The invasive spotted-wing Drosophila (Diptera, Drosophilidae) has been found in the city of São Paulo (Brazil). Revista Brasileira de Entomologia. v.58, n.4, p.371–375, 2014. DOI: http://dx.doi.org/10.1590/S0085-56262014000400004.

WOODRING, J.L.; KAYA, H.K. Steinernematid and heterorhabditid nematodes: A Handbook of Biology and Techniques. Arkansas, Fayetteville: Arkansas Agricultural Experiment Station. Southern Cooperative Series Bulletin. V.331, 1988. 30p.

WOLTZ, J.M.; DONAHUE, K.M.; BRUCK, D.J.; LEE, J.C. Efficacy of commercially available predators, nematodes and fungal entomopathogens for augmentative control of Drosophila suzukii. Journal Applied Entomology. v.139, n.10, p.759–770, 2015. DOI: http://dx.doi.org/10.1111/jen.12200.

WHITE, G.F. A method for obtaining infective nematode larvae from cultures. Science. v.66, 302– 303, 1927. DOI: http://dx.doi.org/10.1126/science.66.1709.302-a.




DOI: https://doi.org/10.30945/rcr-v21i2.2725

Apontamentos

  • Não há apontamentos.